Anti-Aging ( EpiDermFT™ )
APPLICATION NOTE
In Vitro Evaluation of Cosmetic Ingredients and Formulations
for Anti-Aging Efficacy using EpiDermFTTM
Objective
To evaluate the anti-aging efficacy of topically applied cosmetic ingredients and formulations by measuring the expression of ECM components in the EpiDermFT in vitro human skin model.
Methods
EpiDermFT tissues (Figure 1) were produced in the MatTek Corporation GMP tissue production facility. 25μl of each formulation was applied topically for 24 hrs. After treatment, EpiDermFT tissues were processed for total RNA isolation. Total RNA was utilized for gene expression analysis by quantitative PCR.
Figure 1. Histology of EpiDermFT. H&E stained cross-section showing that the tissue morphology of EpiDermFT closely parallels that of normal human skin. The epidermis contains basal, spinous, granular keratinocytes and stratum corneum layers and the dermis contains viable fibroblasts. (400x)
Results
EpiDermFT tissues treated with Formulation A showed significant increases in Collagen 1A1, Collagen 3A1 and Elastin gene expression. Tissues treated with Formulation B showed significant increases in COL3A1 expression (Figure 2).
Figure 2. Gene Expression of EpiDermFT. Genes of interest are compared to untreated controls. Data are presented as the average fold regulation of experimental replicates.
Conclusion
Evaluation of ECM components by quantitative PCR in the EpiDermFT in vitro human skin model can be used in efficacy and claims substantiation studies.
Additional anti-aging capabilities with the EpiDermFT tissue model include gene expression analysis (e.g. MMPs, TIMPs, etc.), protein analysis (Pro-collagen Type I C-terminal Peptide, Hyaluronic Acid, Elastin, MMPs, TIMPs, Inflammatory Mediators, etc.), and histological analysis, amongst others.
The Model
MatTek's EpiDermFT system consists of normal, human-derived epidermal keratinocytes and normal, human-derived dermal fibroblasts which have been cultured to form a multilayered, highly differentiated model of the human dermis and epidermis. Cultured on specially prepared cell culture inserts using serum-free culture medium, EpiDermFT attains levels of differentiation at the cutting edge of in vitro skin technology. For more information on the EpiDermFT tissue model, click here.
The Endpoints
Gene Expression Analysis
- Collagens
- Elastin
- Fibronectin
- MMPs
- TIMPs
- Inflammatory Mediators
Protein Analysis
- Pro-collagen Type I
- Hyaluronic Acid
- Elastin
- MMPs
- TIMPs
- Inflammatory Mediators
-
Histological Analysis
Technical References
634. UNDERSTANDING METABOLIC PATHWAYS FOR SKIN ANTI-AGING.
Osborne, R., Mullins, L.A., and Jarrold, B.B. P&G Beauty & Grooming, The Procter & Gamble Company, Miami Valley Innovation Center, Cincinnati, Ohio USA. J. of Drugs in Dermatol, 8, 7(Supplement), (2009).
596. DC DETOXDEFENSE™ STIMULATES SKIN’S OWN SOD ANTIOXIDANT PROTECTION AND DETOXIFICATION.
Distinctive Cosmetic Ingredients, LLC, South Plainfield, NJ, USA.
549. AccelaFuze™ PEPTIDE INFUSION THERAPY.
Allan, H., Patterson, M. Woodford Medical Aesthetics, Danbury, Essex, UK.
539. ANTIOXIDANT CAPACITY OF 3D HUMAN SKIN EPIDERM MODEL: EFFECTS ON SKIN MOISTURIZERS.
Grazul-Bilska1,2, A.T., Bilski1,2, J.J., Redmer1,2, D.A., Reynolds1,2, L.P., Abdullah3,4, K.M. and Abdullah3,4, A. 1Department of Animal Sciences, 2Cell Biology Center, North Dakota State University, Fargo. 3Department of Surgery, School of Medicine, University of North Dakota, Grand Forks and 4Plastic Surgery Institute, Fargo, ND, USA. Inter. J. of Cosmetic Science, 31, 201-208, (2009).
518. IN VITRO SKIN BIOMARKER RESPONSES TO A NEW ANTI-AGING PEPTIDE, PAL-KT.
Osborne1, R., Mullins1, L.A., Jarrold1, B.B., Binder1, R.L., Mondon2, P., Lintner2, K. 1P&G Beauty & Grooming and Global Biotechnology, Cincinnati, Ohio USA. 2Sederma, France.
385. USE OF EPIDERM FULL THICKNESS TISSUE FOR ANTI-AGING STUDIES.
Gruber, V., et al. Arch Personal Care Products L.P., South Plainfield, NJ and BioInnovation Laboratories, Inc., McKinney, TX.
www.MatTek.co.kr
