Irritation ( EpiGingival )

The Model

MatTek's EpiGingival tissues consist of normal, human-derived epithelial cells. The tissues, which are cultured on specially prepared cell culture inserts using serum free medium, attain levels of differentiation on the cutting edge of in vitro cell culture technology. Morphologically, the tissue models closely parallel native gingival human tissues, thus providing a useful in vitro means to assess irritancy, disease and other basic oral biology phenomena. For more information on the EpiGingival tissue model, click here.

The Method

Request the full protocol here.

1. Transfer tissues from agarose to assay medium

2. Incubate (37 ±1°C, 5 ±1% CO₂, 95% RH) for 60 ±5 min

3. Transfer tissues to fresh assay medium

4. Dose tissues apically each with 40 μl TS (diluted 1:1 in ultrapureH₂O), PC, or NC

5. Expose 1, 4 and 18 hrs (37 ±1°C, 5 ±1% CO₂, 95% RH)

6. Stop exposure by rinsing in DPBS

7. Collect medium for cytokine analysis (optional)

8. Blot tissues and perform MTT assay

9. Read OD in a plate spectrophotometer at 550-570nm

The Endpoints

MTT Tissue Viability Assay

Cytokine Release (optional)

Technical References

438. ORGANOTYPIC HUMAN ORAL TISSUE MODELS FOR TOXICOLOGICAL STUDIES.    

Klausner¹, M., Ayehunie¹, S., Breyfogle¹, B.A., Wertz², P.W., Bacca³, L., Kubilus¹, J. ¹MatTek Corporation, Ashland, MA 01721, United States, ²University of Iowa, Iowa City, IA 52242, United States, ³Procter and Gamble Company, Mason, OH 45040, United States. Toxicology in Vitro, 21, 938-949 (2007).

379. EPIGINGIVAL™ (GIN-100) TISSUE MODELS FOR ORAL IRRITATION STUDIES.    

Breyfogle¹,B., Kubilus¹, J., Dale², B., Wertz³, P. ¹MatTek Corporation, Ashland, MA, USA, ²University of Washington, Seattle, WA, USA, ³University of Iowa, Iowa City, IA, USA. Presented at 5th World Congress, Berlin, Germany, August, (2005).

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