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(프로토콜) Absorption (EpiGingival)
 

 
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Absorption ( EpiGingival )

 

The Model

MatTek's EpiGingival tissues consist of normal, human-derived epithelial cells. The tissues, which are cultured on specially prepared cell culture inserts using serum free medium, attain levels of differentiation on the cutting edge of in vitro cell culture technology. Morphologically, the tissue models closely parallel native gingival human tissues, thus providing a useful in vitro means to assess irritancy, disease and other basic oral biology phenomena. For more information on the EpiGingival tissue model, click here.

 

The Method

Request the full protocol here.

  1. Transfer tissues from agarose to assay medium

  2. Incubate (37 ±1°C, 5 ±1% CO2, 95% RH) 1 h

  3. Transfer tissues to receiver solution 

  4. Equilibrate tissues to permeation tempurature for 15 min

  5. Apply permeant solution to apical surface of tissues

  6. Collect, store and replace receiver solution every 30 - 60 min for the duration of the study

  7. Determine average flux and calculate the permeability coefficient (Kp)

Technical References

 

672. RETENTION OF O-CYMEN-5-OL AND ZINC ON RECONSTRUCTED HUMAN GINGIVAL TISSUE FROM A TOOTHPASTE FORMULATION.     

Yang, J., Deol, G. and Myangar, N.  GlaxoSmithKline Consumer Healthcare, Weybridge, UK.  Inter. Dental Journal, 61 (Suppl. 3): 41-45, 2011.

 

399. CHARACTERIZATION AND TESTING OF NEW BUCCAL AND GINGIVAL TISSUE MODELS.    

Kubilus1, J., Ayehunie1, S., Breyfogle1, B., Dale2, B., Kimball2, J., Wertz3, P., Klausner1, M. 1MatTek Corporation, Ashland, MA, USA; 2University of Washington, Seattle, USA; 3University of Iowa, Iowa City, USA. Poster presented at American Association for Dental Research and American Dental Education Association annual meeting, Orlando, FL, March 8-11 (2006).


 

 

 

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