Allergenicity Testing

( Dendritic Cells )

 

The Model

MatTek has developed a new method for generating DC from CD34+ progenitor cells (hematopoietic stem cells) harvested from umbilical cord blood. This improved method generates an average of >400 million DC from 1 million starting progenitor CD34+ cells. The DC express CD1a, HLA-DR, CD80, CD86, CD40, CD54, CD11c, CD206 (mannose receptor), and unlike monocyte-derived DC, the DC express BIRBECK GRANULES. The DC also express TLR-1, TLR-2, TLR-3, TLR-4, TLR-6, TLR-7 and TLR-9.The DC are phenotypically similar to their in vivo counterparts, and their in vitro function in immunological reactions mimics their in vivo behavior. These cells have a longer life span in culture and can be used in a broad range of immunology, virology and other functional studies. For more information on Dendritic Cells, click here.

Technical References

646. PLASMACYTOID DENDRITIC CELL-BASED ASSAY AS AN IN VITRO ALTERNATIVE FOR CHEMICAL ALLERGENICITY SCREENING.

Ayehunie, S., Child, M., Spratt, M., Klausner, M. MatTek Corporation, Ashland, MA, USA.  Presented at SOT 2011 Abstract # 87.

449. PLASMACYTOID DENDRITIC CELLS AS A TOOL FOR TESTING ALLERGENICITY OF CHEMICALS.

Ayehunie, S., Snell, M., Hayden, P.J., Klausner, M., and Sheasgreen, J. MatTek Corporation, Ashland, MA 01721. Society of Toxicology 46th Annual Meeting, Charlotte, NC, (2007). Abstract #1146. The Toxicologist, 96, 1, 238 (2007).

311. HARVEST, PROLIFERATION, AND FUNCTIONAL TESTING OF HUMAN DENDRITIC CELLS.

Ayehunie, S., Lamore, S., Bellavance, K., and Klausner, M. MatTek Corporation, Ashland, MA. Poster presented at the American Association of Pharmaceutical Scientists (AAPS), Salt Lake City, UT, October 26-30, (2003).

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