Microbial Infection
( Dendritic Cells )
The Model
MatTek has developed a new method for generating DC from CD34+ progenitor cells (hematopoietic stem cells) harvested from umbilical cord blood. This improved method generates an average of >400 million DC from 1 million starting progenitor CD34+ cells. The DC express CD1a, HLA-DR, CD80, CD86, CD40, CD54, CD11c, CD206 (mannose receptor), and unlike monocyte-derived DC, the DC express BIRBECK GRANULES. The DC also express TLR-1, TLR-2, TLR-3, TLR-4, TLR-6, TLR-7 and TLR-9.The DC are phenotypically similar to their in vivo counterparts, and their in vitro function in immunological reactions mimics their in vivo behavior. These cells have a longer life span in culture and can be used in a broad range of immunology, virology and other functional studies. For more information on Dendritic Cells, please click here.
Technical References
721. HUMAN INTESTINAL DENDRITIC CELLS DECREASE CYTOKINE RELEASE AGAINST SALMONELLA INFECTION IN THE PRESENCE OF LACTOBACILLUS PARACASEI UPON TLR ACTIVATION.
Bermudez-Brito1, M., Munoz-Quezada1, S., Gomez-Llorente1, C., Matencio2, E., Bernal2, M.J., Romero2, F., Gil1, A. 1Institute of Nutrition and Food Technology Jose Mataix, Biomedical Research Centre, Department of Biochemistry and Molecular Biology II, University of Granada, Granada, Spain, 2Hero Institute for Infant Nutrition, Hero Spain, Alcantarilla, Murcia, Spain. PLOS ONE, 7, 8, (2012).
451. ACTIVATED CD34-DERIVED LANGERHANS CELLS MEDIATE TRANSINFECTION WITH HUMAN IMMUNODEFICIENCY VIRUS.
Fahrbach1, K.M., Barry1,2, S.M., Ayehunie3, S., Lamore3, S., Klausner3, M. and Hope1, T.J. 1Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611; 2Department of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago, Chicago, Illinois 60612; and 3MatTek Corporation, 200 Homer Avenue, Ashland, Massachusetts 01721. J. of Virology, 81, (13), 6858-6868, (2007).
440. IN VITRO DERIVED DENDRITIC CELLS TRANS-INFECT CD4 T CELLS PRIMARILY WITH SURFACE-BOUND HIV-1 VIRIONS.
Cavrois1, M., Neidleman1, J., Kreisberg1,2, J.F., Greene1,2,3, W.C. 1Gladstone Institute of Virology and Immunology, University of California San Francisco, San Francisco, California, United States of America, 2Department of Medicine, University of California San Francisco, San Francisco, California, United States of America, 3Department of Microbiology and Immunology, University of California San Francisco, San Francisco, California, United States of America. PLoS Pathogens, 3, (1), 0038-0045, (2007).
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