The EpiOcular^TM Model

주문은 최소 1개월 전에 하셔야 합니다.

Features

• Cornea-Like Three Dimensional Structure
• Quantifiable, Objective Endpoints
• Highly Reproducible
• Excellent correlation - in vivo to in vitro test results
• "Sub-Draize" product testing possible
• Extensive number of materials, products tested
• Topical Testing - Undiluted, Water Insoluble Materials
• Easily Handled Cell Culture Inserts
• Completely Serum-Free Media System
• Cost Effective Alternative to Animal Testing

MatTek's EpiOcular™ corneal model consists of normal, human-derived epidermal keratinocytes which have been cultured to form a stratified, squamous epithelium similar to that found in the cornea. The epidermal cells, which are cultured on specially prepared cell culture inserts using serum free medium, differentiate to form a multi-layered structure which closely parallels the corneal epithelium.

The EpiOcular tissue model exhibits in vivo-like morphological and growth characteristics which are uniform and highly reproducible. EpiOcular consists of highly organized basal cells which progressively flatten out as the apical surface of the tissue is approached, analogous to the normal in vivo corneal epithelium. EpiOcular is mitotically and metabolically active and releases many of the pro-inflammatory agents (cytokines) known to be important in ocular irritation and inflammation.

The protocols for using the EpiOcular System are clear and straightforward. EpiOcular has been utilized with several common tests of cytotoxicity and irritancy, including MTT, IL-1α, PGE2, LDH, and sodium fluorescein permeability. Technicians find EpiOcular's rigid substrate design easy to handle in routine repetitive testing environments and scientists find that they are able to perform discriminating tests due to the model's high level of reproducibility. In fact, "Sub-Draize" testing is possible with EpiOcular, allowing quantifiable discrimination among mild, milder, and mildest product formulations.

Various industrial and toxicology laboratories are actively seeking alternatives to whole animal testing. Cosmetic, household product, pharmaceutical and petrochemical companies have initiated in vitro toxicology testing to evaluate their raw materials and final product formulations. A growing body of data indicates that EpiOcular effectively provides a non-animal means to assess ocular irritation and other toxicological issues.

For all of these reasons, the EpiOcular model provides a predictive, morphologically relevant in vitro means to assess ocular irritancy.

	HISTOLOGY: EpiOcular vs. Rabbit Cornea Formalin Fixed, Paraffin Embedded, H&E Stained EpiOcular: Magnification 360x Rabbit Cornea: Magnification 360x

	OCL-200 Prediction Equation ET-50 (min) Figure 1:  Determination of EpiOcular prediction model -- Correlation of MTT ET-50 results to Draize rabbit eye data.

ECETOC Materials (Figure 1)* ET-50 avg (min) Actual Draize (MMAS) 1 Benzalkonium Chloride (1%) 5.9 45.3 2 Benzalkonium Chloride (5%) 1.0 83.8 3 Benzalkonium Chloride (10%) 1.1 108.0 4 Cetyl Pyridinium Bromide (0.1%) >240 2.7 5 Cetyl Pyridinium Bromide (1%) 30.1 36.0 6 Cetyl Pyridinium Bromide (10%) 9.0 89.7 7 Glycerol >240 1.7 8 Propylene Glycol >240 1.3 9 Sodium Dodecyl Sulfate (3%) 9.0 16.0 10 Sodium Dodecyl Sulfate (15%) 5.1 59.2 11 Sodium Dodecyl Sulfate (30%) 2.1 60.5 12 Sodium Hydroxide (1%) 2.3 25.8 13 Sodium Hydroxide (10%) 1.0 108.0 14 Tri-chloro acetic acid (3%) 155.1 6.7 15 Tri-chloro acetic acid (30%) 1.0 106.0 16 Triton X-100 (1%) 36.7 1.7 17 Triton X-100 (5%) 5.3 33.1 18 Triton X-100 (10%) 2.5 68.7 19 Tween 20 (100%) >240 4.0           Surfactants (Figure 1)*     20 Cetrimonium chloride 116.9 6.7 21 Cocamide DEA >240 0.0 22 Disodium cocoamphodipropionate 11.2 15.3 23 Na2-ricinoleadmido MEA sulfosuccinate 108.9 0.0 24 Sodium cocoamphopropionate 7.1 45.0 25 Sodium trideceth sulfate 2.5 33.0 26 Stearalkonium chloride >240 14.0 27 Surfactant blend 19.2 6.0 28 Surfactant blend 40.4 2.7           Consumer Products (Figure 1)*     29 10% fatty alcohol ethoxylate 189.0  3.5 30 Body spray >240  0.0 31 Body/hand Wash 9.1  32.0 32 Body/hand Wash 14.8  35.0 33 Eye Gel/Colorant >240  0.0 34 Eye Gel/Colorant >240 2.0 35 Eye makeup remover (surfactant solution) >240  0.0 36 Face/body Wash >240  2.0 37 Face/body Wash 6.5  25.0 38 Face/body Wash 10.2  40.0 39 Final formulation shampoo 63.1  3.5 40 Final formulation shampoo 31.0  3.9 41 Final formulation shampoo 26.1  4.0 42 Final formulation shampoo 42.1  4.8 43 Final formulation shampoo 29.4  6.6 44 Final formulation shampoo 47.1  8.3 45 Final formulation shampoo 29.1  12.5 46 Final formulation shampoo 9.3  31.6 47 Final formulation shampoo 4.2  32.7 48 Final formulation shampoo 9.0  34.4 49 Hair conditioner >240  2.0 50 Hand/body Lotion >240  2.0 51 Hand/body Lotion >240  2.0 52 Hand/body Lotion >240 3.0 53 Hydroalcohol (air spray) solution 84.1  6.0 54 Lactic acid (3%) >240  0.0 55 Oleic acid >240  2.0 56 Shampoo - baby 30.8 10.0 57 Shampoo - baby 25.7  18.0 58 Shampoo - regular 6.0  30.0 59 Shampoo - regular 8.7  35.0 60 Skin care emulsion >240  0.0

Testing of OCL-200 Prediction Model       In Vivo (MMAS) vs. Predicted Draize In Vivo Draize (MMAS) Figure 2:  Comparison of actual Draize to predicted Draize score based on correlation equation (Figure 1).   Consumer Products (Figure 2)* Predicted Draize Actual Draize (MMAS)         1 Body wash 14.6 16.7 2 Body wash 31.3 44.7 3 Dishwashing liquid 51.3 38.3 4 Hand soap liquid 28.0 24.7 5 Dishwashing liquid 25.2 39.3 6 Facial soap 15.6 9.3 7 Dishwashing liquid 50.8 39.0 8 Dishwashing liquid 60.5 50.3 9 Laundry detergent 37.3 37.3 10 Laundry detergent 1.8 0.7 11 Laundry detergent 33.4 37.7 12 Dishwashing liquid 96.9 37.7 13 Shampoo 6.5 4.0 14 Shampoo 32.9 41.7 15 Shampoo 8.4 3.3 16 Hand soap liquid 18.1 13.3 17 Skin lotion 1.8 0.7 18 Shampoo 46.9 33.7 19 Skin lotion 1.8 0.0 20 Shampoo 39.3 37.7 21 Body wash 41.2 33.0 22 Laundry detergent 1.8 0.7 23 Laundry detergent 39.9 44.0 24 Skin lotion 1.8 0.7 *  All materials diluted to 20% in ultrapure water prior to testing (density > 0.95 g/ml).